Journal: Scientific Reports

Article Title: The DNA Repair Protein OGG1 Protects Against Obesity by Altering Mitochondrial Energetics in White Adipose Tissue

doi: 10.1038/s41598-018-33151-1

Figure Lengend Snippet: Adipose tissue metabolism is altered in Ogg1 Tg mice. Metabolic parameters were determined in eWAT of WT and Ogg1 Tg mice (n = 6, unless otherwise indicated). Expression of genes regulating lipid catabolism ( a ), inflammation ( c ), and mitochondrial energy metabolism ( f ) was quantitated by qRT-PCR using gene-specific primers. Plasma FFA were determined in HFD-fed and overnight-fasted mice (n = 5) using a colorimetric kit ( b ). Macrophage infiltration into eWAT sections was determined by immunohistochemistry using an antibody against the macrophage protein F4/80, and staining intensity, quantified in ImageJ, is shown in the top left corner of images ( d ). In addition, protein levels of the inflammatory protein NFκB was determined by IHC using an antibody against NFκB, followed by counterstaining with DAPI ( e ). Protein levels of SIRT-1 and PGC-1α were determined by SDS-PAGE ( f ), and β-actin was utilized as a loading control. Plasma leptin ( g ) and adiponectin ( h ) levels were determined by ELISA. Data are expressed as average ± SEM. *p < 0.05 vs. WT mice; # p < 0.05 vs. chow-fed mice. Acox, acyl-CoA oxidase, Atgl, adipose triglyceride lipase, Cpt-1, carnitine palmitoyl transferase-1, eWAT, epididymal white adipose tissue, FFA, free fatty acids, Hsl, hormone-sensitive lipase, Pgc-1α, PPAR-gamma coactivator-1 alpha, Pparα, peroxisome proliferator activated receptor-alpha, Sirt1, sirtuin 1.

Article Snippet: Plasma insulin (Millipore, St. Charles, MO), plasma leptin (RayBiotech, Norcross, GA) and plasma adiponectin (Abcam, Cambridge, MA) were measured by ELISA.

Techniques: Expressing, Quantitative RT-PCR, Immunohistochemistry, Staining, SDS Page, Enzyme-linked Immunosorbent Assay

Journal: BMC Research Notes

Article Title: Pilot study on the effects of a 2-week hiking vacation at moderate versus low altitude on plasma parameters of carbohydrate and lipid metabolism in patients with metabolic syndrome

doi: 10.1186/s13104-015-1066-3

Figure Lengend Snippet: Mean values for blood lipid parameters before (T 0 ) and after (T 1 ) the training program in the low (N=6) and moderate (N=8) altitude study groups

Article Snippet: Immunoenzyme analysis (sandwich ELISA) was used to measure concentrations of total plasma leptin (DGR, Siemens BEP) and plasma adiponectin (BioVendor, Siemens BEP).

Techniques: